506 research outputs found

    The roles of aldehyde dehydrogenases (ALDHs) in the PDH bypass of Arabidopsis

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    <p>Abstract</p> <p>Background</p> <p>Eukaryotic aldehyde dehydrogenases (ALDHs, EC 1.2.1), which oxidize aldehydes into carboxylic acids, have been classified into more than 20 families. In mammals, Family 2 ALDHs detoxify acetaldehyde. It has been hypothesized that plant Family 2 ALDHs oxidize acetaldehyde generated via ethanolic fermentation, producing acetate for acetyl-CoA biosynthesis via acetyl-CoA synthetase (ACS), similar to the yeast pathway termed the "pyruvate dehydrogenase (PDH) bypass". Evidence for this pathway in plants has been obtained from pollen.</p> <p>Results</p> <p>To test for the presence of the PDH bypass in the sporophytic tissue of plants, Arabidopsis plants homozygous for mutant alleles of all three Family 2 ALDH genes were fed with <sup>14</sup>C-ethanol along with wild type controls. Comparisons of the incorporation rates of <sup>14</sup>C-ethanol into fatty acids in mutants and wild type controls provided direct evidence for the presence of the PDH bypass in sporophytic tissue. Among the three Family 2 ALDHs, one of the two mitochondrial ALDHs (ALDH2B4) appears to be the primary contributor to this pathway. Surprisingly, single, double and triple ALDH mutants of Arabidopsis did not exhibit detectable phenotypes, even though a Family 2 ALDH gene is required for normal anther development in maize.</p> <p>Conclusion</p> <p>The PDH bypass is active in sporophytic tissue of plants. Blocking this pathway via triple ALDH mutants does not uncover obvious visible phenotypes.</p

    Research on Enterprises Reputation Evaluation Based on B2B Transaction Platform

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    Based on traders’ goods, service,the way of transaction and transaction time, the paper establishes the B2B traders’ reputation evaluation index system, and applies the Analytic Hierarchy Process to give weight. Using the reputation evaluation model, the paper takes into account evaluators’ own reputation, transaction amount, transaction time and other factors, dynamically adjusting reputation value and generating the reputation value of traders which is as the basis for the traders to choose the trading partners, and to promote the development of B2B e-commerce.Key words: B2B; Traders; Enterprises reputation; Evaluatio

    Static stiffness modeling and sensitivity analysis for geared system used for rotary feeding

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    The positioning accuracy of rotary feed system under load greatly depends on the static stiffness of mechanical transmission system. This paper proposes a unified static stiffness model of rotary feed system with geared transmission system. Taking the torsional stiffness of transmission shaft and mesh stiffness of gear pairs into account, the motion equations of the whole transmission system are presented. Based on the static equilibrium, a unified expression for the relationship between torsional angles of two adjacent elements is derived. Then a unified static stiffness model is presented. Furthermore, analytical expressions for sensitivity analysis of the static stiffness on the individual element’s stiffness and design parameters are derived. The presented model is verified by a traditional model, and a good agreement is obtained. The influence of phase angle of meshing gear pairs on the resultant static stiffness is investigated. An example transmission system is employed to perform the sensitivity analysis and the results are analyzed. The proposed model provides an essential tool for the design of rotary feed system satisfying requirement of static stiffness

    Multiobjective Transmission Network Planning considering the Uncertainty and Correlation of Wind Power

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    In order to consider the uncertainty and correlation of wind power in multiobjective transmission network expansion planning (TNEP), this paper presents an extended point-estimation method to calculate the probabilistic power flow, based on which the correlative power outputs of wind farm are sampled and the uncertain multiobjective transmission network planning model is transformed into a solvable deterministic model. A modified epsilon multiobjective evolutionary algorithm is used to solve the above model and a well-distributed Pareto front is achieved, and then the final planning scheme can be obtained from the set of nondominated solutions by a fuzzy satisfied method. The proposed method only needs the first four statistical moments and correlation coefficients of the output power of wind farms as input information; the modeling of wind power is more precise by considering the correlation between wind farms, and it can be easily combined with the multiobjective transmission network planning model. Besides, as the self-adaptive probabilities of crossover and mutation are adopted, the global search capabilities of the proposed algorithm can be significantly improved while the probability of being stuck in the local optimum is effectively reduced. The accuracy and efficiency of the proposed method are validated by IEEE 24 as well as a real system

    Towards Runtime Customizable Trusted Execution Environment on FPGA-SoC

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    Processing sensitive data and deploying well-designed Intellectual Property (IP) cores on remote Field Programmable Gate Array (FPGA) are prone to private data leakage and IP theft. One effective solution is constructing Trusted Execution Environment (TEE) on FPGA-SoCs (FPGA System on Chips). Researchers have integrated this type TEE with Trusted Platform Module (TPM)-based trusted boot, denoted as FPGA-SoC tbTEE. But there is no effort on secure and trusted runtime customization of FPGA-SoC TEE. This paper extends FPGA-SoC tbTEE to build Runtime Customizable TEE (RCTEE) on FPGA-SoC by additive three major components (our work): 1) CrloadIP, which can load an IP core at runtime such that RCTEE can be adjusted dynamically and securely; 2) CexecIP, which can not only execute an IP core without modifying the operating system of FPGA-SoC TEE, but also prevent insider attacks from executing IPs deployed in RCTEE; 3) CremoAT, which can provide the newly measured RCTEE state and establish a secure and trusted communication path between remote verifiers and RCTEE. We conduct a security analysis of RCTEE and its performance evaluation on Xilinx Zynq UltraScale+ XCZU15EG 2FFVB1156 MPSoC

    Effect of JUNCAO-cultivated Ganoderma lucidum spent mushroom substrate-hot water extract on immune function in mice

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    Purpose: To evaluate the effect of JUNCAO-cultivated Ganoderma lucidum spent mushroom substratehot water extract (SMSG-HWE) on murine immunity.Methods: Five groups of mice (n = 10) received water with 0.00, 0.14, 0.28, 0.84, or 1.68 g/kg of SWSG-HWE, respectively, orally for 30 days. Various biochemical parameters of serum and tissues, including spleen and thymus indices, were determined were determined for the miceResults: The following markers were significantly higher in the 0.84 g/kg SMSG-HWE group than in the control group (all p &lt; 0.05): splenic lymphocyte proliferation, a marker of cell-mediate immunity; dinitrofluorobenzene-induced delayed hypersensitivity; and the number of haemolytic plaque-forming cells, as a marker of humoral immunity. Phagocytic rate, which evaluates mononuclear-macrophage function as a marker of innate immunity, was significantly higher in both the 0.84 g/kg HWE and 1.68 g/kg SWSG-HWE groups, while phagocytic index was significantly higher in the 0.28 g/kg SWSG-HWE group, compared to the control group (all p &lt; 0.05). Natural killer cell activity also was significantly enhanced in the 0.84 g/kg and 1.68 g/kg SWSG-HWE groups (p &lt; 0.05).Conclusion: These findings indicate that SWSG-HWE enhances murine immune function, and may be suitable as a potential additive in animal feed.Keywords: Hot water extract, Cell-mediated immune function, Concanavalin A, Mononuclearmacrophage function, Haemolytic plaque, Humoral immunity, Innate immunity, Animal feed additiv

    Research on Post-Evaluation of Oilfield Ground Engineering Project

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    Based on the research results of the scholars from domestic and overseas, we construct a evaluation index system for post- evaluation of oilfield ground engineering project focusing on the characteristics of the ground engineer project and combining the characteristics of the development of oilfield. The system includes 3 primary indicators, such as pre-project, 8 secondary indicators and 35 tertiary indicators. Multistage fuzzy evaluation with the combination of quantitative analysis qualitative analysis and degree of success method are used for Post-evaluation of oilfield ground engineering project which may lead to the reference of the decision-making of investment and management for oilfield ground engineering project.Key words: Oilfield ground engineering; Postevaluation; Evaluation index; Multistage fuzzy evaluatio
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